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KMID : 0613820090190010117
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Journal of Life Science
2009 Volume.19 No. 1 p.117 ~ p.122
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Antioxidative Effect of Angelica acutiloba Kitagawa Ethanol Extract
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Kim Ah-Ra
Lee Jae-Joon
Lee Myong-Ryeol
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Abstract
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This study was conducted to investigate the antioxidant effect of 80% ethanol extracts from Angelica acutiloba Kitagawa (A. acutiloba Kitagawa) in vitro. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, n-butanol and water. Antioxidative activities of different fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical generation, Rancimat test, thiobarbituric acid (TBA) value, nitrite scavenging activity, inhibition of lipid peroxidation and peroxide value (POV) in linoleic acid in comparison with the commercial antioxidant butylated hydroxy toluene (BHT). Antioxidant activities of n-hexane fraction of Angelico acutiloba Kitagawa ethanol extract were the highest among fractions and were a little less than that of BHT. Nitrite scavenging activity showed the most remarkable effect at pH 12. These results suggest that ethanol extracts of A. acutiloba Kitagawa can be used in natural antioxidant source.
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KEYWORD
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Angelica acutiloba Kitagawa, antioxidant activity, nitrite scavenging activity
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